Welcome to the CoursePlus Web site for SPECIAL TOPICS IN BIOCHEMISTRY: X-RAY CRYSTALLOGRAPHY-A HANDS ON WORKSHOP (120.861.01), a course offered by the Department of Biochemistry and Molecular Biology at the Johns Hopkins Bloomberg School of Public Health.
Enables students to carry out all key steps to successfully solve and refine a protein crystal structure. Theoretical aspects are followed by application to various problem sets. Topics include tricks for data collection, data processing and collection. Touches upon all standard techniques such as molecular replacement, SAD phasing and MAD phasing, both in theory and then applied in practical context with previously collected data. Identification of unknown ligand densities and model refinement lead to the last part of preparing publication quality figures using PyMol.
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Grading Policy: Students are evaluated on their participation in classroom discussions and also on completion of assigned homework and tutorial data.
Grading Restrictions: Letter grade