Welcome to the CoursePlus Web site for NUCLEIC ACID CHEMISTRY (120.613.01), a course offered by the Department of Biochemistry and Molecular Biology at the Johns Hopkins Bloomberg School of Public Health.
Discusses nucleic acid structure, and also describes techniques for manipulating and analyzing nucleic acids, including gel electrophoresis, PCR, and DNA sequencing. Reviews methods used to synthesize nucleosides, nucleotides and oligonucleotides, and chemical reactions that lead to modifications of nucleic acids. Additional topics include: nucleic acid molecular beacons and molecular wires; antisense and antigene oligonucleotides; nucleic acid nanostructures and "machines", and nucleic acid aptamers.
This course will prepare you to be able to do the following:
- Design schemes to synthesize nucleosides and oligonucleotides
- Predict the products of reactions between nucleic acids and various chemical agents, ionizing and ultraviolet radiation
- Understand the design and function of antisense, antigene and siRNAs
- Understand the design and synthesis of nucleic acid nanostructures and machines
- Understand how nucleic acid aptamers are applied to biological problems
Mon Wed 3:30 PM to 4:50 PM
Organic Chemistry and Biochemistry and/or Molecular Biology
PhD or MHS students interested in biochemical sciences
Grading Policy: Final exam at end of term.
Grading Restrictions: Letter grade