NUCLEIC ACID CHEMISTRY Syllabus
Course Learning Objectives
This course will prepare you to be able to do the following:
- Design schemes to synthesize nucleosides and oligonucleotides
- Predict the products of reactions between nucleic acids and various chemical agents, ionizing and ultraviolet radiation
- Understand the design and function of antisense, antigene and siRNAs
- Understand the design and synthesis of nucleic acid nanostructures and machines
- Understand how nucleic acid aptamers are applied to biological problems
Discusses nucleic acid structure, and also describes techniques for manipulating and analyzing nucleic acids, including gel electrophoresis, PCR, and DNA sequencing. Reviews methods used to synthesize nucleosides, nucleotides and oligonucleotides, and chemical reactions that lead to modifications of nucleic acids. Additional topics include: nucleic acid molecular beacons and molecular wires; antisense and antigene oligonucleotides; nucleic acid nanostructures and "machines", and nucleic acid aptamers.
PhD or MHS students interested in biochemical sciences
Methods of Assessment
Grading Policy: Final exam at end of term.
Grading Restrictions: Letter grade
Organic Chemistry and Biochemistry and/or Molecular Biology
Academic Ethics Code
The code, discussed in the Policy and Procedure Memorandum for Students, March 31, 2002, will be adhered to in this class:
Students enrolled in the Bloomberg School of Public Health of The Johns Hopkins University assume an obligation to conduct themselves in a manner appropriate to the University's mission as an institution of higher education. A student is obligated to refrain from acts which he or she knows, or under the circumstances has reason to know, impair the academic integrity of the University. Violations of academic integrity include, but are not limited to: cheating; plagiarism; knowingly furnishing false information to any agent of the University for inclusion in the academic record; violation of the rights and welfare of animal or human subjects in research; and misconduct as a member of either School or University committees or recognized groups or organizations.
Disability Support Services
If you are a student with a documented disability who requires an academic accommodation, please contact Betty H. Addison in the Office of Student Life Services: firstname.lastname@example.org, 410-955-3034, or 2017 E. Monument Street.